Detector performs an important function while in the finalization of any analytical method. Commonly almost all of the organic/drug molecules are aromatic or unsaturated in nature, that has an absorption inside the UV–vis area. This will come as an advantage in quantifying and analyzing the molecules and its affiliated impurities.Additionally, a

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The inner diameter (ID) of the HPLC column is an important parameter that influences the detection sensitivity and separation selectivity in gradient elution. What's more, it determines the amount of analyte that can be loaded on to the column.In several instances, baseline separation from the peaks is usually realized only with gradient elution an